Author: Baldwin, Don A.; Feldman, Michael; Alwine, James C.; Robertson, Erle S.
Title: Metagenomic Assay for Identification of Microbial Pathogens in Tumor Tissues Document date: 2014_9_16
ID: xlqdn0c7_8
Snippet: Pilot assays using Agilent reference human DNA showed median probe intensities of over 750 fluorescence units for probes to human sequences, around 17 fluorescence units for nonhuman specific probes on PathoChip v2a, and 120 fluorescence units for nonhuman conserved probes on PathoChip v2b (experiment 1, Table 1 ). These assays identified 6,360 probes with fluorescence values of Ͼ150 that would apparently be able to hybridize to human DNA and we.....
Document: Pilot assays using Agilent reference human DNA showed median probe intensities of over 750 fluorescence units for probes to human sequences, around 17 fluorescence units for nonhuman specific probes on PathoChip v2a, and 120 fluorescence units for nonhuman conserved probes on PathoChip v2b (experiment 1, Table 1 ). These assays identified 6,360 probes with fluorescence values of Ͼ150 that would apparently be able to hybridize to human DNA and were therefore removed from consideration for generation of the PathoChip v3 design, which combined the unique and conserved probe sets (Fig. 1B) .
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