Author: Willemsen, Anouk; Zwart, Mark P
Title: On the stability of sequences inserted into viral genomes Document date: 2019_11_14
ID: vv5gpldi_17
Snippet: The loss of sequences inserted into baculovirus genomes is not only due to the formation of DIs. When an origin of replication that is enriched in DI genomes was removed, baculovirus genomic stability at high MOIs increased as no DIs were observed. Strikingly, inserted foreign sequences were still rapidly lost (Pijlman, van Schinjndel, and Vlak 2003) , showing that rapid DI generation is not the only impediment to the stability of inserted genes......
Document: The loss of sequences inserted into baculovirus genomes is not only due to the formation of DIs. When an origin of replication that is enriched in DI genomes was removed, baculovirus genomic stability at high MOIs increased as no DIs were observed. Strikingly, inserted foreign sequences were still rapidly lost (Pijlman, van Schinjndel, and Vlak 2003) , showing that rapid DI generation is not the only impediment to the stability of inserted genes. Addition of endogenous viral sequences-homologous repeat regions important for baculovirus replication-to inserted sequences promoted the stability of insertions (Pijlman et al. 2004) , highlighting the importance of the genomic context for insert stability.
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