Mice Lacking Expression of Secondary Lymphoid Organ Chemokine Have Defects in Lymphocyte Homing and Dendritic Cell Localization

Gunn, Michael D.; Kyuwa, Shigeru; Tam, Carmen; Kakiuchi, Terutaka; Matsuzawa, Akio; Williams, Lewis T.; Nakano, Hideki

We also examined the migration of splenic DCs upon activation. Mouse spleen normally contains a population of interdigitating DCs located within the T cell zone and a separate population of DCs located in marginal zone bridg- ing channels and red pulp (30, 36, 37) . Treatment of mice with LPS has been shown to cause a rapid decrease in the number of DCs located in bridging channels and red pulp and a simultaneous increase in the number of interdigitating DCs (38) . This has led to the hypothesis that splenic DCs outside the T cell zone represent an immature population that migrates to the T cell zone upon activation. 6 h after intraperitoneal injection of LPS, spleens of Ï©/Ï© mice demonstrated intense staining of DCs within T cell zones but few DCs in bridging channels or red pulp (Fig. 5 C) . By contrast, the number of interdigitating cells in the splenic T cell zones of plt mice did not increase after LPS treatment. Most DCs remained scattered throughout the red pulp (Fig. 5 D) .