Author: Willemsen, Anouk; Zwart, Mark P
Title: On the stability of sequences inserted into viral genomes Document date: 2019_11_14
ID: vv5gpldi_15
Snippet: Non-human adenoviruses can be used as alternative vaccine vectors, providing several advantages such as a limited host range and restricted replication in non-host species. By using bovine adenovirus type 3, a variety of antigens and cytokines were successfully expressed in vivo (Ayalew et al. 2015) . The stability of bovine adenovirus type 1 was tested by inserting the EYFP marker and subsequently passaging the recombinant virus in cell culture .....
Document: Non-human adenoviruses can be used as alternative vaccine vectors, providing several advantages such as a limited host range and restricted replication in non-host species. By using bovine adenovirus type 3, a variety of antigens and cytokines were successfully expressed in vivo (Ayalew et al. 2015) . The stability of bovine adenovirus type 1 was tested by inserting the EYFP marker and subsequently passaging the recombinant virus in cell culture (Ren et al. 2018) . Although replication of this recombinant virus was less efficient than the wild-type virus, the inserted EYFP was stable.
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