Selected article for: "gene expression and RNA virus"
Author: Willemsen, Anouk; Zwart, Mark P
Title: On the stability of sequences inserted into viral genomes
  • Document date: 2019_11_14
    • ID: vv5gpldi_59
    Snippet: What is particularly interesting about the genome organization of VSV and other ssRNA(À) viruses is that promoter proximal genes are more efficiently expressed than promoter distal ones (Iverson and Rose 1981; Wertz, Perepelitsa, and Ball 1998; Pesko et al. 2015) . The efficiency of expression of the inserted gene (and therewith the strength of the immune response) can be controlled (Tokusumi et al. 2002; Roberts et al. 2004 ). However, insertin.....
    Document: What is particularly interesting about the genome organization of VSV and other ssRNA(À) viruses is that promoter proximal genes are more efficiently expressed than promoter distal ones (Iverson and Rose 1981; Wertz, Perepelitsa, and Ball 1998; Pesko et al. 2015) . The efficiency of expression of the inserted gene (and therewith the strength of the immune response) can be controlled (Tokusumi et al. 2002; Roberts et al. 2004 ). However, inserting a foreign gene close to the promoter also can also reduce the expression of downstream vector genes (Skiadopoulos et al. 2002) , which in turn can negatively affect virus transcription and RNA replication (Wertz, Moudy, and Ball 2002; Zhao and Peeters 2003) . These empirical observations again show that the site of the insert plays an important role in recombinant vector stability.

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