ONTOGENY OF MACROPHAGE RESISTANCE TO MOUSE HEPATITIS IN VIVO AND IN VITRO

Gallily, Ruth; Warwick, Anne; Bang, Frederik B.

Mice.--The delay in onset of both disease in infant mice and the time of killing of the cultures suggested that some adaptation of the virus was taking place in the CsH animals and cultures. In three sets of experiments it appeared that following one transfer of MHV(PRI) to infant CsH mice (4-5 days old) the virus became adapted to adult CsH mice. About half of the adult CsH mice were killed when injected with a 1:100 dilution of virus prepared from 10% fiver suspension of an infant CsH mouse. In contrast, undiluted and diluted MHV(PRI) failed to kill CsH adult mice. This preparation of MHV will be referred to as MHHV(CsH). Table V summarizes the mortality of infant C~T-I mice, adult C~H mice, and adult PRI mice after injection of different passages of MHHV(CsH) in CJ-I infant mice. However, at no time did virus prepared from the infected livers of adult CsH mice have the ability to kill other adult Cai.i mice. The PRI mice remained susceptible to this adult CsH preparation, and all mice so infected were killed (Table VI) . Table VII shows the survival time of infant CsH mice, adult CsH mice, and adult PRI mice after MHV(CsH) injection. Infant CsH mice died 2 days after MHV(CsHH) injection, while 7 days elapsed before MHV(PRI) killed CsH mice of the same age (Table II) .