Author: Wang, Yanqun; Liu, Di; Shi, Weifeng; Lu, Roujian; Wang, Wenling; Zhao, Yanjie; Deng, Yao; Zhou, Weimin; Ren, Hongguang; Wu, Jun; Wang, Yu; Wu, Guizhen; Gao, George F.; Tan, Wenjie
Title: Origin and Possible Genetic Recombination of the Middle East Respiratory Syndrome Coronavirus from the First Imported Case in China: Phylogenetics and Coalescence Analysis Document date: 2015_9_8
ID: x6sjdglm_2
Snippet: Characterization of genome. With informed consent and the approval of the ethical committee of the National Institute of Viral Disease Control and Prevention, China Center for Disease Control and Prevention (CDC), nasopharyngeal swabs were collected and used for RNA extraction, followed by reverse transcription PCR and genome sequencing. Through both Sanger and Ion Torrent sequencing, the full-length virus genome (30,144 bp) of ChinaGD01 was obta.....
Document: Characterization of genome. With informed consent and the approval of the ethical committee of the National Institute of Viral Disease Control and Prevention, China Center for Disease Control and Prevention (CDC), nasopharyngeal swabs were collected and used for RNA extraction, followed by reverse transcription PCR and genome sequencing. Through both Sanger and Ion Torrent sequencing, the full-length virus genome (30,144 bp) of ChinaGD01 was obtained and deposited in GenBank (accession no. KT006149). Over 2,000,000 paired-end reads were quality trimmed and processed to remove human genome sequences. Nonhuman reads were assembled into contigs by CLC Genomic Workbench and aligned against representative sequences of MERS-CoV. No nucleotide insertions or deletions were observed in the genome.
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