Title: Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network Document date: 1991_12_2
ID: syyi2ysq_9
Snippet: To induce the synthesis of free GAG chains, PC12 cells were heated with the xylose analogue 4methylumbelliferyl S-D-xyloside (xyloside) . Xyloside, dissolved in DMSO at 500 mM, was routinely used at a final concentration of 1 mM . PC12 cells were preincubated for 1 .5 h in growth medium, for 0.5 h in sulfate-free DMEM and then pulse labeled for 5 min with fresh sulfate-free DMEM containing 1 mCi/ml [ 35 S]sulfate, all in the presence of 1 mM xylo.....
Document: To induce the synthesis of free GAG chains, PC12 cells were heated with the xylose analogue 4methylumbelliferyl S-D-xyloside (xyloside) . Xyloside, dissolved in DMSO at 500 mM, was routinely used at a final concentration of 1 mM . PC12 cells were preincubated for 1 .5 h in growth medium, for 0.5 h in sulfate-free DMEM and then pulse labeled for 5 min with fresh sulfate-free DMEM containing 1 mCi/ml [ 35 S]sulfate, all in the presence of 1 mM xyloside . In pulse-chase experiments, pulse labeling was followed by incubation in DMEM containing 1 mM xyloside and twice the normal concentration of unlabeled sulfate.
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