Selected article for: "human receptor and monoclonal antibody"
Author: Glennie, S; Gritzfeld, J F; Pennington, S H; Garner-Jones, M; Coombes, N; Hopkins, M J; Vadesilho, C F; Miyaji, E N; Wang, D; Wright, A D; Collins, A M; Gordon, S B; Ferreira, D M
Title: Modulation of nasopharyngeal innate defenses by viral coinfection predisposes individuals to experimental pneumococcal carriage
  • Document date: 2015_4_29
    • ID: st475jw7_30
    Snippet: Epithelial polymeric immunoglobulin receptor and platelet-activating factor receptor expression by flow cytometry. Stimulated (inflamed) and nonstimulated (noninflamed) cells were scraped from the wells after washing with cold PBS. Cell suspensions were incubated with platelet-activating factor receptor, human monoclonal antibody, and polymeric immunoglobulin receptor rabbit polyclonal antibody (both from Cambridge Bioscience, Cambridge, UK) at 4.....
    Document: Epithelial polymeric immunoglobulin receptor and platelet-activating factor receptor expression by flow cytometry. Stimulated (inflamed) and nonstimulated (noninflamed) cells were scraped from the wells after washing with cold PBS. Cell suspensions were incubated with platelet-activating factor receptor, human monoclonal antibody, and polymeric immunoglobulin receptor rabbit polyclonal antibody (both from Cambridge Bioscience, Cambridge, UK) at 4 1C for 15 min. Anti-human PE-conjugate and anti-rabbit FITC-conjugate antibodies were added for 15 min at 4 1C. Cells were washed and stored at 4 1C before acquisition using a BD LSR II flow cytometer. A total of 10,000 gated events were recorded. PE goat anti-mouse IgG2a and FITC donkey anti-rabbit IgG (both from Biolegend, London, UK) were used as isotype controls.

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