Author: Menachery, Vineet D.; Eisfeld, Amie J.; Schäfer, Alexandra; Josset, Laurence; Sims, Amy C.; Proll, Sean; Fan, Shufang; Li, Chengjun; Neumann, Gabriele; Tilton, Susan C.; Chang, Jean; Gralinski, Lisa E.; Long, Casey; Green, Richard; Williams, Christopher M.; Weiss, Jeffrey; Matzke, Melissa M.; Webb-Robertson, Bobbie-Jo; Schepmoes, Athena A.; Shukla, Anil K.; Metz, Thomas O.; Smith, Richard D.; Waters, Katrina M.; Katze, Michael G.; Kawaoka, Yoshihiro; Baric, Ralph S.
Title: Pathogenic Influenza Viruses and Coronaviruses Utilize Similar and Contrasting Approaches To Control Interferon-Stimulated Gene Responses Document date: 2014_5_20
ID: s3zeppze_28
Snippet: Calu3 cells were utilized as previously described (5-7). Viral titrations and propagation of SARS-CoV, MERS-CoV, and influenza virus were performed in VeroE6 and MDCK cells using standard methods. Wild-type (WT) icSARS-CoV was derived from the Baric laboratory's infectious clone (ic) constructs (53) . MERS-CoV (EMC 2012 strain) was kindly provided by Bart L. Haagmans, Erasmus Medical Center, Rotterdam, Netherlands, via Material Transfer Agreement.....
Document: Calu3 cells were utilized as previously described (5-7). Viral titrations and propagation of SARS-CoV, MERS-CoV, and influenza virus were performed in VeroE6 and MDCK cells using standard methods. Wild-type (WT) icSARS-CoV was derived from the Baric laboratory's infectious clone (ic) constructs (53) . MERS-CoV (EMC 2012 strain) was kindly provided by Bart L. Haagmans, Erasmus Medical Center, Rotterdam, Netherlands, via Material Transfer Agreement. Influenza A/California/04/2009 (H1N1-09), influenza A/Vietnam/1203/2004 (H5N1-VN1203), and H5N1-VN1203 mutants (NS1trunc124, PB1-F2del, and PB2-K627E) were derived from a plasmid-based reversegenetic system and subsequently amplified in MDCK cells (54) . Detailed information about construction of mutant viruses is included in the supplemental material (see Text S1).
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