Author: Cao, Rui-Yuan; Xu, Yong-fen; Zhang, Tian-Hong; Yang, Jing-Jing; Yuan, Ye; Hao, Pei; Shi, Yi; Zhong, Jin; Zhong, Wu
Title: Pediatric Drug Nitazoxanide: A Potential Choice for Control of Zika Document date: 2017_2_3
ID: sbf9qi6g_17
Snippet: Zika virus with 10 μM nitazoxanide or 10 μM tizoxanide were bound to the Vero cells for 1 hour at 4°C, then washed 3 times with PBS, and ZIKV RNA levels were determined by quantitative RT-PCR (qRT-PCR) immediately after binding [10] . Zika virus with 10μM nitazoxanide or 10 μM tizoxanide were incubated for 2 hours at 4°C, then washed 3 times with PBS, and changed into 10% FBS DMEM; 72 hours later, the cells were harvested for ZIKV RNA detec.....
Document: Zika virus with 10 μM nitazoxanide or 10 μM tizoxanide were bound to the Vero cells for 1 hour at 4°C, then washed 3 times with PBS, and ZIKV RNA levels were determined by quantitative RT-PCR (qRT-PCR) immediately after binding [10] . Zika virus with 10μM nitazoxanide or 10 μM tizoxanide were incubated for 2 hours at 4°C, then washed 3 times with PBS, and changed into 10% FBS DMEM; 72 hours later, the cells were harvested for ZIKV RNA detection with qRT-PCR assay [11] .
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