Author: Chang, Stewart T.; Sova, Pavel; Peng, Xinxia; Weiss, Jeffrey; Law, G. Lynn; Palermo, Robert E.; Katze, Michael G.
Title: Next-Generation Sequencing Reveals HIV-1-Mediated Suppression of T Cell Activation and RNA Processing and Regulation of Noncoding RNA Expression in a CD4(+) T Cell Line Document date: 2011_9_20
ID: zyzgk2z3_5
Snippet: We report here the effects of HIV-1 infection on the expression of polyadenylated RNAs in a CD4 Ï© T cell line. Using NGS, we detected small but significant changes in host gene expression affecting T cell function that coincided with the initiation of viral RNA production at 12 h postinfection (hpi). These changes intensified near peak viral replication at 24 hpi when a multitude of other host processes were disrupted as well. In addition, by us.....
Document: We report here the effects of HIV-1 infection on the expression of polyadenylated RNAs in a CD4 Ï© T cell line. Using NGS, we detected small but significant changes in host gene expression affecting T cell function that coincided with the initiation of viral RNA production at 12 h postinfection (hpi). These changes intensified near peak viral replication at 24 hpi when a multitude of other host processes were disrupted as well. In addition, by using NGS, we observed the dramatic expansion of viral mRNA expression and detected new viral splice events occurring during viral replication and differential expression of noncoding RNA species, including microRNA host genes. These findings provide an unprecedented and comprehensive view into the transcriptomelevel changes that occur within T cells infected with replicating HIV.
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