Phylogeny of bovine norovirus in Egypt based on VP2 gene

Mohamed, Fakry F.; Ktob, Gamelat K.F.; Ismaeil, Mohamed E.A.; Ali, Ahmed A.H.; Goyal, Sagar M.

Twenty-nine fecal specimens were collected aseptically from sporadic cases of diarrheic calves in Sharkia province of Egypt between late 2016 and early 2017. Calves had diarrhea, dehydration, and weakness with different degrees. The age of calves ranged from 3 weeks to 3 months. No deaths were observed at the time of sample collection. According to the case history, animals were not vaccinated against locally-identified enteric viruses, including bovine rotavirus (BRV) and bovine viral diarrhea virus (BVDV). All samples were diluted in phosphate buffer saline (10×) and centrifuged at 2500×g for 10 min. The viral RNA was extracted from the supernatant fluid of each sample (140 µL) by following the manufacturer's instructions of the QIAamp Viral RNA Mini Kit (QIAGEN, Valencia, CA, USA).