n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils

Wu, Beibei; Wang, Liyin; Jiang, Lili; Dong, Lili; Xu, Fengli; Lu, Yili; Jin, Jiahui; Wang, Zhanyue; Liang, Guang; Shan, Xiaoou

As previously reported, the present study observed that LPS stimulation decreased the expression of CXCR1, CXCR2 and CD62L on neutrophils. In addition to being effector cells involved in the acute phase of the inflammatory response, neutrophils are also important in the resolution of inflammation. The failure of neutrophils to migrate can lead to an inability to control infection due to weakened neutrophil phagocytic and bactericidal abilities (26) . CXCR1 and CXCR2 are the major chemokine receptors on neutrophils. Chemokine receptors regulate the migration of neutrophils to the site of infection to assist in controlling invading pathogens and protecting the body. Duerschmied et al (27) demonstrated that survival following LPS-induced endotoxic shock improved upon the promotion of neutrophil recruitment during acute inflammation. n-butanol extract was found to have significant antiseptic abilities in our previous study. However, whether the extract affected neutrophil migration remained unclear. In the present study, it was demonstrated that n-butanol extract prevented the downregulation of CXCR1, CXCR2 and CD62L.