Author: Ding, Peiyang; Zhang, Teng; Li, Yafei; Teng, Man; Sun, Yaning; Liu, Xiao; Chai, Shujun; Zhou, Enmin; Jin, Qianyue; Zhang, Gaiping
Title: Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2 Document date: 2017_7_24
ID: upb97on4_29
Snippet: At 35 and 49 dpi, the lymphocytes were isolated from the spleens of 3 mice from each group using mouse lymphocyte separation medium (Solarbio, Beijing, People's Republic of China) and resuspended at 5×10 5 cells/mL in RPMI-1640 complete medium containing 10% FBS. The splenocytes were seeded into 96-well plates at 100 μL per well and stimulated with 100 μL complete medium containing Cap protein (20 μg/mL) or 100 μL complete medium alone. Afte.....
Document: At 35 and 49 dpi, the lymphocytes were isolated from the spleens of 3 mice from each group using mouse lymphocyte separation medium (Solarbio, Beijing, People's Republic of China) and resuspended at 5×10 5 cells/mL in RPMI-1640 complete medium containing 10% FBS. The splenocytes were seeded into 96-well plates at 100 μL per well and stimulated with 100 μL complete medium containing Cap protein (20 μg/mL) or 100 μL complete medium alone. After incubation for 48 h, the proliferative responses were determined using CCK-8. The stimulation index (SI) was calculated using the following formula: SI = (the mean of
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