Author: Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María
Title: Laboratory Detection of Respiratory Viruses by Automated Techniques Document date: 2012_11_30
ID: ted64zo4_36
Snippet: The monitoring of accumulating amplicons in real time is achieved by labelling of primers, oligonucleotide probes or amplicons with fluorophores. These emit fluorescent signals that accumulate during the exponential phase of the amplification step, which is recorded by the instrument. The signal is related to the amount of amplicon present during each cycle and will increase as the amount of specific amplicon increases. Various chemistries have b.....
Document: The monitoring of accumulating amplicons in real time is achieved by labelling of primers, oligonucleotide probes or amplicons with fluorophores. These emit fluorescent signals that accumulate during the exponential phase of the amplification step, which is recorded by the instrument. The signal is related to the amount of amplicon present during each cycle and will increase as the amount of specific amplicon increases. Various chemistries have been used for real-time PCR assays, i.e. Taqman ® probes, FRET probes, intercalating dyes as Sybr Green I, molecular beacons, scorpions oligonucleotides, etc [35] .
Search related documents:
Co phrase search for related documents- amplification step and molecular beacon: 1
- amplification step and oligonucleotide probe: 1
- amplification step and PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- amplification step and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- amplification step and real time PCR assay: 1, 2, 3, 4, 5, 6
- exponential phase and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
Co phrase search for related documents, hyperlinks ordered by date