Selected article for: "Multiplex PCR assay and PCR assay"
Author: Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María
Title: Laboratory Detection of Respiratory Viruses by Automated Techniques
  • Document date: 2012_11_30
    • ID: ted64zo4_45
    Snippet: Luminex xTAG ® RVP was the first panel that received the FDA approval. The version RVP Fast has shortened the initial time of 8-10 h required to accomplish the whole procedure. The RVP Fast assay incorporates multiplex RT-PCR using primers with universal tags. The amplified product is hybridized to a bead array conjugated to specific probes. Detection is performed using a streptavidin-Rphycoerythrin conjugate. Each bead population detects a uniq.....
    Document: Luminex xTAG ® RVP was the first panel that received the FDA approval. The version RVP Fast has shortened the initial time of 8-10 h required to accomplish the whole procedure. The RVP Fast assay incorporates multiplex RT-PCR using primers with universal tags. The amplified product is hybridized to a bead array conjugated to specific probes. Detection is performed using a streptavidin-Rphycoerythrin conjugate. Each bead population detects a unique viral target by hybridisation to a specific anti-tag. A signal (median fluorescence intensity) is generated for each bead population [40] . These fluorescence values are analyzed by using xTAG ® data analysis software for RVP Fast (TDAS RVP Fast) to establish the presence or absence of viral targets in each sample. A recent version of the xTAG RVP Fast panel (RVP v2) is CE marked and identifies 19 viral types and subtypes, which now includes Flu A(H1N1)pdm09.

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