Author: Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María
Title: Laboratory Detection of Respiratory Viruses by Automated Techniques Document date: 2012_11_30
ID: ted64zo4_46
Snippet: A preliminary evaluation of RVP v2 has demonstrated a higher recovery of RV and 100% specificity compared to "in-house" real-time PCRs. No competition was observed in samples spiked with combinations of 2 RV. Limit of detection for A(H1N1)pdm09, Flu A H3N2 and RSV was calculated with replicates of 10-fold dilutions of positive samples. Semi-quantitation of viral loads was accomplished by measurement of the C T value observed in parallel "in-house.....
Document: A preliminary evaluation of RVP v2 has demonstrated a higher recovery of RV and 100% specificity compared to "in-house" real-time PCRs. No competition was observed in samples spiked with combinations of 2 RV. Limit of detection for A(H1N1)pdm09, Flu A H3N2 and RSV was calculated with replicates of 10-fold dilutions of positive samples. Semi-quantitation of viral loads was accomplished by measurement of the C T value observed in parallel "in-house" real-time PCRs. For this purpose, mean C T of 30 obtained from the original RT-PCR was chosen. RVP v2 detected RSV and H3N2 down to a 10 -2 dilution of the sample, and A(H1N1)pdm09 was detected down to a 10 -3 dilution whereas positive "in-house" PCR reached the 10 -2 dilution [41] .
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