Author: Huo, Caiyun; Xiao, Jin; Xiao, Kai; Zou, Shumei; Wang, Ming; Qi, Peng; Liu, Tianlong; Hu, Yanxin
Title: Pre-Treatment with Zirconia Nanoparticles Reduces Inflammation Induced by the Pathogenic H5N1 Influenza Virus Document date: 2020_1_30
ID: wqzve7i5_17
Snippet: Madin-Darby canine kidney (MDCK) cells were cultured in Dulbecco's modified Eagle medium (DMEM) (HyClone Laboratories, Logan, UT, USA) containing 10% fetal bovine serum (HyClone Laboratories), 100 U/mL penicillin, and 100 μg/mL streptomycin. Right lung homogenates from individual mice were prepared and diluted 10-fold in DMEM and added to a monolayer of MDCK cells in semisolid agar containing 0.5 μg/mL trypsin-tolylsulfonyl phenylalanyl chlorom.....
Document: Madin-Darby canine kidney (MDCK) cells were cultured in Dulbecco's modified Eagle medium (DMEM) (HyClone Laboratories, Logan, UT, USA) containing 10% fetal bovine serum (HyClone Laboratories), 100 U/mL penicillin, and 100 μg/mL streptomycin. Right lung homogenates from individual mice were prepared and diluted 10-fold in DMEM and added to a monolayer of MDCK cells in semisolid agar containing 0.5 μg/mL trypsin-tolylsulfonyl phenylalanyl chloromethyl ketone (TPCK) (Sigma, Beijing, China). The cultures were incubated at 37°C and 5% CO 2 for 60-72 h, fixed, and stained with 1% crystal violet. Plaque-forming units (PFUs) were then counted.
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