Selected article for: "nucleotide identity and reference sequence"
Author: Huang, Guohong; Yu, Deshan; Zhu, Zhen; Zhao, Hai; Wang, Peng; Gray, Gregory C.; Meng, Lei; Xu, Wenbo
Title: Outbreak of febrile respiratory illness associated with human adenovirus type 14p1 in Gansu Province, China
  • Document date: 2013_5_22
    • ID: t74wq8ru_14
    Snippet: All the 11 clinical specimens were separately inoculated into A549 cells. A characteristic adenovirus-like CPE was observed in A549 cells from six of the throat swab specimens ( Table 2) . Of the 11 adenovirus-positive samples, the hypervariable region (HVR1-6) of the hexon gene was amplified, and BLAST sequence analysis revealed 100% identical with HAdV-14p1 (strain isolate Portland/2971/ z2007, USA HAdV-14p1; GenBank accession number FJ841901; .....
    Document: All the 11 clinical specimens were separately inoculated into A549 cells. A characteristic adenovirus-like CPE was observed in A549 cells from six of the throat swab specimens ( Table 2) . Of the 11 adenovirus-positive samples, the hypervariable region (HVR1-6) of the hexon gene was amplified, and BLAST sequence analysis revealed 100% identical with HAdV-14p1 (strain isolate Portland/2971/ z2007, USA HAdV-14p1; GenBank accession number FJ841901; Table 2 ). Amplifications and nucleotide identity of Gansu2011-01-11 strains were 100% identical to the HAdV-14p1 reference sequence (GenBank accession number: FJ841901). CDC public health officials concluded that all the patients were infected with the same HAdV-14p1 virus. One (Gansu2011-01 strain) of the 6 HAdV isolates was selected for further sequence studies. The complete hexon, fiber, and E1A genes were amplified, sequenced, and compared with the sequences of HAdV-B2 species found in GenBank

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