Author: Willemsen, Anouk; Zwart, Mark P
Title: On the stability of sequences inserted into viral genomes Document date: 2019_11_14
ID: vv5gpldi_70
Snippet: Concordantly, the eGFP sequence was inserted into MLV under different configurations (i.e. site of insertion and flanking sequence), and the reporter gene was deleted upon extended cell culture (Duch et al. 2004 ). The stability was improved by decreasing the length of sequence repeats flanking the inserted sequence, however, eventually eGFP was always (partially or completely) deleted (Duch et al. 2004) . In another study, transgenes of differen.....
Document: Concordantly, the eGFP sequence was inserted into MLV under different configurations (i.e. site of insertion and flanking sequence), and the reporter gene was deleted upon extended cell culture (Duch et al. 2004 ). The stability was improved by decreasing the length of sequence repeats flanking the inserted sequence, however, eventually eGFP was always (partially or completely) deleted (Duch et al. 2004) . In another study, transgenes of different sizes (GFP, hph, pac) were inserted into MLV. Deletions were always observed, where the deletion dynamics depended on the size of the insert and preferred sites of recombination were detected (Logg et al. 2001) .
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