ONTOGENY OF MACROPHAGE RESISTANCE TO MOUSE HEPATITIS IN VIVO AND IN VITRO

Gallily, Ruth; Warwick, Anne; Bang, Frederik B.

The results are presented in Table III and Text- fig. 1 . The data show that the susceptibility of CsH mouse liver macrophages to MHV(PRI) decreased with the increase in the age of the donor animal. Susceptibility was indicated when about 75-100 % of cells were destroyed. While 73 % of the cultures derived from 1-day-old liver were susceptible, only 7 % of the cultures derived from 5-day-old liver and none of those from the 8-day-old liver were susceptible to this virus. Table III ) were resistant. This maturation of resistance apparently took place in the animal. Experiments therefore were undertaken to determine if susceptible fiver macrophages become resistant after being cultured in vitro for different lengths of time; i.e., will maturation or ontogeny of resistance proceed in vitro? Pieces of C~-I liver from 1-day-old mice were placed on collagen as before. MVH(PRI) was added to the tubes after 3, 7, and 10 days had elapsed. The results are summarized in Table IV . Continued growth in vitro did not increase the resistance of the liver macrophages to MHVfPRI) and indeed may have decreased resistance.