Author: Parvez, Mohammad K.; Tabish Rehman, Md.; Alam, Perwez; Al-Dosari, Mohammed S.; Alqasoumi, Saleh I.; Alajmi, Mohammed F.
Title: Plant-derived antiviral drugs as novel hepatitis B virus inhibitors: Cell culture and molecular docking study Document date: 2018_12_26
ID: xibqsjib_20
Snippet: To rule out the inhibitory effect of the tested antiviral compounds on host-synthesized proteins, luciferase assay was performed as described previously (Al-Dosari and Parvez, 2018) . Briefly, HepG2.2.15 cells were transfected with Renilla-luciferase plasmid (pRL-TK; Promega, USA) using FuGENE6 (Promega, USA) in a 48-well culture plate, following treatment (10 lg/ml; in triplicate) with all compounds, including DMSO (0.1%) as untreated control. A.....
Document: To rule out the inhibitory effect of the tested antiviral compounds on host-synthesized proteins, luciferase assay was performed as described previously (Al-Dosari and Parvez, 2018) . Briefly, HepG2.2.15 cells were transfected with Renilla-luciferase plasmid (pRL-TK; Promega, USA) using FuGENE6 (Promega, USA) in a 48-well culture plate, following treatment (10 lg/ml; in triplicate) with all compounds, including DMSO (0.1%) as untreated control. After 2 days of incubation (i.e., day 3 post-transfection), cell lysates were prepared and luciferase expression was measured (Luciferase Reporter Assay System; Promega, USA), and data were presented in relation to the negative control.
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