Author: SUZUKI, Kazuya; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens Document date: 2016_10_30
ID: tg4qupbm_7
Snippet: It was previously reported that FLK-N3 cells show CPE after infection with MVV but not with caprine arthritis encephalitis virus though their biological properties are very similar [10] . The Iwate strain did not grow in the FLK-N3 cell line in the present study. These phenomena may be related to the history of in vitro passages of each virus. Equine infectious anemia virus, another lentivirus, well adapted to primary horse leukocyte culture, was.....
Document: It was previously reported that FLK-N3 cells show CPE after infection with MVV but not with caprine arthritis encephalitis virus though their biological properties are very similar [10] . The Iwate strain did not grow in the FLK-N3 cell line in the present study. These phenomena may be related to the history of in vitro passages of each virus. Equine infectious anemia virus, another lentivirus, well adapted to primary horse leukocyte culture, was found to be infective for cultivated horse kidney cells [9] . If the FLK-N3 cell line was infected with the Iwate strain after a long-term subculture in primary FGL cells, then an FLK-N3 cell line persistently infected with MVV might be established.
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