Author: Zhang, Xiao-Ai; Lu, Qing-Bin; Wo, Ying; Zhao, Jin; Huang, Dou-Dou; Guo, Chen-Tao; Xu, Hong-Mei; Liu, En-Mei; Liu, Wei; Cao, Wu-Chun
Title: Prevalence and genetic characteristics of Saffold cardiovirus in China from 2009 to 2012 Document date: 2015_1_9
ID: pwlbrxn3_5
Snippet: Laboratory detection. All collected samples were tested for SAFV using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay targeting the 59 untranslated region (UTR) as previously described 11 . All samples with positive reactions were further amplified using nested RT-PCR targeting the 59-UTR, which generated a 540-bp amplicon 11 . A patient was considered to be infected with SAFV when positive detection was obtained in any .....
Document: Laboratory detection. All collected samples were tested for SAFV using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay targeting the 59 untranslated region (UTR) as previously described 11 . All samples with positive reactions were further amplified using nested RT-PCR targeting the 59-UTR, which generated a 540-bp amplicon 11 . A patient was considered to be infected with SAFV when positive detection was obtained in any type of the samples. The viral protein (VP) 1 gene of all positive samples were amplified using nested RT-PCR approach as previously described 26 .
Search related documents:
Co phrase search for related documents- chain reaction and laboratory detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and nest RT PCR: 1
- chain reaction and positive detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and positive reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and positive reaction sample: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and positive sample: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and reverse transcription: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and reverse transcription polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and RT PCR approach: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- chain reaction and RT PCR chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and sample type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- collect sample and positive detection: 1, 2
- collect sample and positive sample: 1, 2, 3
- collect sample and real time: 1, 2
- collect sample and reverse transcription: 1, 2, 3
- collect sample and reverse transcription polymerase: 1, 2, 3
- collect sample and RT PCR chain reaction: 1
- collect sample and sample type: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date