Author: LI, Xunliang; LI, Pengchong; CAO, Liyan; BAI, Yunyun; CHEN, Huijie; LIU, He; REN, Xiaofeng; LI, Guangxing
Title: Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model Document date: 2019_9_2
ID: rme6b022_14
Snippet: Lymphocytes were collected from the peripheral blood or spleen of the experimental animals, and lymphocyte proliferation assays were performed using isolated MCs from the blood and spleen, as previously described [5, 10, 27] . The MCs were diluted to 10 5 cells/50 µl Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Waltham, MA, U.S.A.) and added to each well of 96-well plates. Concanavalin A (Sigma, Munich, Germany) and purified recomb.....
Document: Lymphocytes were collected from the peripheral blood or spleen of the experimental animals, and lymphocyte proliferation assays were performed using isolated MCs from the blood and spleen, as previously described [5, 10, 27] . The MCs were diluted to 10 5 cells/50 µl Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Waltham, MA, U.S.A.) and added to each well of 96-well plates. Concanavalin A (Sigma, Munich, Germany) and purified recombinant TGEV-S1 protein (20 µg/ml) were added as stimulating agents for 72 hr. The cells were treated with methylthiazolyldiphenyl-tetrazolium bromide (MTT, 10 µl/well) for 4 hr and the reaction was terminated with dimethyl sulfoxide (DMSO). The viability of cells was calculated by reading the absorbance at 490 nm wavelength.
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