Author: LI, Xunliang; LI, Pengchong; CAO, Liyan; BAI, Yunyun; CHEN, Huijie; LIU, He; REN, Xiaofeng; LI, Guangxing
Title: Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model Document date: 2019_9_2
ID: rme6b022_30
Snippet: Changes in the frequencies of CD4 + and CD8 + cells in the blood and spleen from experimental animals were evaluated using flow cytometry. The number of CD4 + and CD8 + cells in the peripheral blood is shown in Fig. 2C . The number of CD8 + cells in the pVAX1-(TGEV-S1) plus pVAX1-(pIL-12) group was significantly higher (P<0.01) than that in the other groups at 14 and 28 dpi. The number of CD4 + cells in the peripheral blood at 28 dpi was signific.....
Document: Changes in the frequencies of CD4 + and CD8 + cells in the blood and spleen from experimental animals were evaluated using flow cytometry. The number of CD4 + and CD8 + cells in the peripheral blood is shown in Fig. 2C . The number of CD8 + cells in the pVAX1-(TGEV-S1) plus pVAX1-(pIL-12) group was significantly higher (P<0.01) than that in the other groups at 14 and 28 dpi. The number of CD4 + cells in the peripheral blood at 28 dpi was significantly higher (P<0.01) in pVAX1-(TGEV-S1) and pVAX1-(TGEV-S1) plus pVAX1-(pIL-12) groups than in the control groups. A similar trend was observed in the number of CD4 + and CD8 + cells in the spleen, as shown in Fig. 2D . At 28 dpi, the pVAX1-(TGEV-S1) plus pVAX1-(pIL-12) group had significantly higher (P<0.01) number of CD4 + and CD8 + cells than other groups.
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