Selected article for: "PCR amplification and real time PCR amplification"

Author: Goktas, Safak; Sirin, Mumtaz Cem
Title: Prevalence and Seasonal Distribution of Respiratory Viruses During the 2014 - 2015 Season in Istanbul
  • Document date: 2016_8_22
  • ID: uhjftet5_3
    Snippet: Molecular diagnostic methods allow the identification of a wide range of viral and bacterial pathogens within hours, with excellent sensitivity and specificity. Multiplex real-time polymerase chain reaction (PCR) assays provide simultaneous amplification of several viruses or bacteria in a single reaction and make it easy to detect the common causative agents of ARTIs (5, 7) . In addition, these tests are able to detect emerging respiratory virus.....
    Document: Molecular diagnostic methods allow the identification of a wide range of viral and bacterial pathogens within hours, with excellent sensitivity and specificity. Multiplex real-time polymerase chain reaction (PCR) assays provide simultaneous amplification of several viruses or bacteria in a single reaction and make it easy to detect the common causative agents of ARTIs (5, 7) . In addition, these tests are able to detect emerging respiratory viruses, such as human metapneumovirus (HMPV), human coronavirus (HCoV) NL63, HCoV HKU1 and human bocavirus (HBoV), which are difficult to grow in cell cultures (3, (8) (9) (10) .

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