Author: Huang, G Khai Lin; Tio, Shio Yen; Caly, Leon; Nicholson, Suellen; Thevarajan, Irani; Papadakis, Georgina; Catton, Mike; Tong, Steven Y C; Druce, Julian
Title: Prolonged Detection of Japanese Encephalitis Virus in Urine and Whole Blood in a Returned Short-term Traveler Document date: 2017_9_27
ID: ymcgs03n_3
Snippet: Diagnostic assays performed on the initial and a repeat CSF (on day 10) were negative using conventional gel-based and realtime multiplex polymerase chain reactions (PCRs) for herpes viruses (herpes simplex 1 and 2 and varicella zoster), enterovirus, pan-flavivirus (Murray Valley encephalitis [MVE], Kunjin, dengue, West Nile, Zika, yellow fever, JEV), respiratory viruses (influenza, respiratory syncytial virus, parainfluenza, human metapneumoviru.....
Document: Diagnostic assays performed on the initial and a repeat CSF (on day 10) were negative using conventional gel-based and realtime multiplex polymerase chain reactions (PCRs) for herpes viruses (herpes simplex 1 and 2 and varicella zoster), enterovirus, pan-flavivirus (Murray Valley encephalitis [MVE], Kunjin, dengue, West Nile, Zika, yellow fever, JEV), respiratory viruses (influenza, respiratory syncytial virus, parainfluenza, human metapneumovirus, picornavirus, adenovirus, coronavirus), meningococcus, and pneumococcus. The conventional pan-flavivirus PCR and JEV real-time PCR performed on plasma were also negative (see the Supplementary Data for detailed methods). HIV and syphilis serology were negative, as were bacterial cultures and screening swabs for Burkholderia pseudomallel.
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