Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer Document date: 2020_1_1
ID: tymoeyoo_27
Snippet: Next, we constructed a prokaryotic expression vector containing SPINK1, successfully purified the protein ( fig. S5F ), and detected its activity. Immunoblotting was performed using purified SPINK1 to confirm PRSS1 inhibition by SPINK1. Reduced proteolytic cleavage was observed in the purified SPINK1 group compared to that in the non-SPINK1 group. Purified SPINK1 significantly inhibited the PRSS1-mediated proteolytic cleavage of mAbs both in vitr.....
Document: Next, we constructed a prokaryotic expression vector containing SPINK1, successfully purified the protein ( fig. S5F ), and detected its activity. Immunoblotting was performed using purified SPINK1 to confirm PRSS1 inhibition by SPINK1. Reduced proteolytic cleavage was observed in the purified SPINK1 group compared to that in the non-SPINK1 group. Purified SPINK1 significantly inhibited the PRSS1-mediated proteolytic cleavage of mAbs both in vitro and in the HT-29 cell culture supernatant (Fig. 4, E to G) . These results all suggest that SPINK1 may be an effective inhibitor of the PRSS1-mediated proteolytic cleavage of cetuximab.
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