Author: Mohamed, Fakry F.; Ktob, Gamelat K.F.; Ismaeil, Mohamed E.A.; Ali, Ahmed A.H.; Goyal, Sagar M.
Title: Phylogeny of bovine norovirus in Egypt based on VP2 gene Document date: 2018_4_13
ID: rah5p1y4_13
Snippet: The PCR products of BNoV-VP2 were purified using a QIAquick PCR purification kit (QIAGEN, Valencia, CA, USA) and sequenced, directly in both directions using the same primers previously used in RT-PCR, at the University of Minnesota Genomics Center (UMGC). Using Sequencher 5.1 software (http://genecodes.com/), contigs were generated using forward and reverse sequences. The obtained sequences were further identified by online nucleotide BLAST tool.....
Document: The PCR products of BNoV-VP2 were purified using a QIAquick PCR purification kit (QIAGEN, Valencia, CA, USA) and sequenced, directly in both directions using the same primers previously used in RT-PCR, at the University of Minnesota Genomics Center (UMGC). Using Sequencher 5.1 software (http://genecodes.com/), contigs were generated using forward and reverse sequences. The obtained sequences were further identified by online nucleotide BLAST tool (http://www.ncbi.nlm.nih.gov/BLAST).
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