Author: Mohamed, Fakry F.; Ktob, Gamelat K.F.; Ismaeil, Mohamed E.A.; Ali, Ahmed A.H.; Goyal, Sagar M.
Title: Phylogeny of bovine norovirus in Egypt based on VP2 gene Document date: 2018_4_13
ID: rah5p1y4_13
Snippet: The PCR products of BNoV-VP2 were purified using a QIAquick PCR purification kit (QIAGEN, Valencia, CA, USA) and sequenced, directly in both directions using the same primers previously used in RT-PCR, at the University of Minnesota Genomics Center (UMGC). Using Sequencher 5.1 software (http://genecodes.com/), contigs were generated using forward and reverse sequences. The obtained sequences were further identified by online nucleotide BLAST tool.....
Document: The PCR products of BNoV-VP2 were purified using a QIAquick PCR purification kit (QIAGEN, Valencia, CA, USA) and sequenced, directly in both directions using the same primers previously used in RT-PCR, at the University of Minnesota Genomics Center (UMGC). Using Sequencher 5.1 software (http://genecodes.com/), contigs were generated using forward and reverse sequences. The obtained sequences were further identified by online nucleotide BLAST tool (http://www.ncbi.nlm.nih.gov/BLAST).
Search related documents:
Co phrase search for related documents- obtained sequence and previously RT PCR: 1, 2, 3
- online nucleotide blast tool and RT PCR: 1
- PCR product and previously RT PCR: 1, 2, 3
- PCR product and purification kit: 1, 2, 3, 4, 5, 6, 7, 8
- PCR product and RT PCR: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57
- PCR product and sequence reverse: 1, 2, 3
- PCR product and sequence reverse forward: 1
- PCR product and Sequencher software: 1
- previously RT PCR and purification kit: 1
- previously RT PCR and QIAquick PCR purification kit: 1
- previously RT PCR and RT PCR: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76
- previously RT PCR primer and RT PCR: 1
- purification kit and QIAquick PCR purification kit: 1, 2, 3, 4, 5, 6, 7, 8, 9
- purification kit and RT PCR: 1, 2, 3, 4, 5, 6, 7
- purification kit and sequence reverse: 1
- QIAquick PCR purification kit and RT PCR: 1
- RT PCR and sequence reverse: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- RT PCR and sequence reverse forward: 1, 2, 3
- RT PCR and Sequencher software: 1
Co phrase search for related documents, hyperlinks ordered by date