Author: Rogers, J.; Schoepp, R.J.; Schröder, O.; Clements, T.L.; Holland, T.F.; Li, J.Q.; Li, J.; Lewis, L.M.; Dirmeier, R.P.; Frey, G.J.; Tan, X.; Wong, K.; Woodnutt, G.; Keller, M.; Reed, D.S.; Kimmel, B.E.; Tozer, E.C.
Title: Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases Document date: 2008_5_13
ID: xkx56h0o_11
Snippet: The cell pellet was resuspended in sonication buffer (0.5 mM DTT, 0.05% Tween 20, 0.1 mg/ml salmon sperm DNA, 10 mg/ml bovine serum albumin (BSA) or 5% milk powder, 50 mM Na-Glutamate, 100 mM ZnCl 2 , 10 mM HEPES, pH 7.4), centrifuged, and again resuspended in 250-500 ml of sonication buffer. The cells were sonicated, centrifuged at 48C at 16 000 r.p.m. for 10 min, and the supernatants removed and kept on ice......
Document: The cell pellet was resuspended in sonication buffer (0.5 mM DTT, 0.05% Tween 20, 0.1 mg/ml salmon sperm DNA, 10 mg/ml bovine serum albumin (BSA) or 5% milk powder, 50 mM Na-Glutamate, 100 mM ZnCl 2 , 10 mM HEPES, pH 7.4), centrifuged, and again resuspended in 250-500 ml of sonication buffer. The cells were sonicated, centrifuged at 48C at 16 000 r.p.m. for 10 min, and the supernatants removed and kept on ice.
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