Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer Document date: 2020_1_1
ID: tymoeyoo_56
Snippet: Total RNA (1 ïg) was extracted and reverse-transcribed with the GoScript Reverse Transcription System. Real-time PCR was performed using QuantiTect SYBR Green PCR Master Mix (Applied Biosystems) in triplicate and analyzed on an ABI 7500 Fast Real-Time PCR system (Applied Biosystems). Human PRSS1, SPINK1, and GAPDH mRNA levels were quantified by reverse transcription PCR (RT-PCR) and real-time qPCR. The PRSS1 primer sequences were as follows:.....
Document: Total RNA (1 ïg) was extracted and reverse-transcribed with the GoScript Reverse Transcription System. Real-time PCR was performed using QuantiTect SYBR Green PCR Master Mix (Applied Biosystems) in triplicate and analyzed on an ABI 7500 Fast Real-Time PCR system (Applied Biosystems). Human PRSS1, SPINK1, and GAPDH mRNA levels were quantified by reverse transcription PCR (RT-PCR) and real-time qPCR. The PRSS1 primer sequences were as follows:
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