Author: Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva
Title: Recent Advances in Biosensor Development for Foodborne Virus Detection Document date: 2017_7_5
ID: sj6zfybb_72
Snippet: An assay based on probes of gold nanoparticles and colorimetric anti-hemagglutinin antibodies is used to detect human H3N2 influenza viruses [69] . Plasmon coupling is used to aggregate the antibody-gold nanoparticle and to release a visible light emission, which is detected by a UV spectrophotometer. The assay is a single step and enzyme-free virus detection method applicable to other virus pathogens. Another assay based on colorimetric hybrid g.....
Document: An assay based on probes of gold nanoparticles and colorimetric anti-hemagglutinin antibodies is used to detect human H3N2 influenza viruses [69] . Plasmon coupling is used to aggregate the antibody-gold nanoparticle and to release a visible light emission, which is detected by a UV spectrophotometer. The assay is a single step and enzyme-free virus detection method applicable to other virus pathogens. Another assay based on colorimetric hybrid gold nanoparticles binding to carbon nanotube surface is used to detect human H3N2 influenza viruses [70] . The nanohybrid architecture is confirmed by transmission electron microscopy, and UV-visible spectrophotometer has a catalytic ability to oxidise tetramethyl-benzidine by H2O2 and yield a blue color in aqueous solution depending on the virus concentration. The assay sensitivity can reach a detection limit of 3.4 PFU/mL which is 380 times more sensitive than the conventional ELISA assay.
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