Title: Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence Document date: 1993_3_1
ID: qt44izzh_45
Snippet: As mentioned above, a fraction of transiently transfected cells expressed the Tac-TGN38 chimeras at the plasma membrane. Almost invariably, this population of cells had visibly higher levels of fluorescence intensity as compared to cells displaying pure TGN staining, consistent with the previous observation that overexpression of TGN38 in CV-I cells results in the appearance of the protein at the cell surface (Luzio et al., 1990) . In a few of th.....
Document: As mentioned above, a fraction of transiently transfected cells expressed the Tac-TGN38 chimeras at the plasma membrane. Almost invariably, this population of cells had visibly higher levels of fluorescence intensity as compared to cells displaying pure TGN staining, consistent with the previous observation that overexpression of TGN38 in CV-I cells results in the appearance of the protein at the cell surface (Luzio et al., 1990) . In a few of the most brightly stained NRK cells expressing T-G-G at the plasma membrane, no endogenous TGN38 staining was visible by immunofluorescence microscopy (data not shown). This phenomenon appeared to occur only in cells with very high levels of protein expression.
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