Selected article for: "primer forward primer and real time"
Author: WU, Hong-Xia; WANG, Hua-Lei; GUO, Xiao-Feng; YANG, Yu-Jiao; MA, Jin-Zhu; WANG, Tie-Cheng; GAO, Yu-Wei; ZHAO, Yong-Kun; YANG, Song-Tao; XIA, Xian-Zhu
Title: Adeno-Associated Viruses Serotype 2-Mediated RNA Interference Efficiently Inhibits Rabies Virus Replication In Vitro and In Vivo
  • Document date: 2013_6_14
    • ID: sj9k4c3i_10
    Snippet: Real-time PCR: NA cells or mouse brains were harvested after RAbV infection, and total RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The RNA was reverse transcribed into cDNA using AMV reverse transcriptase (Promega, Madison, WI, U.S.A.). The levels of N mRNA transcripts were determined by qPCR using brilliant II SYbR Green QPCR Master Mix (Stratagene, La Jolla, CA, U.S.A.) and the following.....
    Document: Real-time PCR: NA cells or mouse brains were harvested after RAbV infection, and total RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The RNA was reverse transcribed into cDNA using AMV reverse transcriptase (Promega, Madison, WI, U.S.A.). The levels of N mRNA transcripts were determined by qPCR using brilliant II SYbR Green QPCR Master Mix (Stratagene, La Jolla, CA, U.S.A.) and the following gene-specific primers: forward primer (1168-1187 in genome) 5'-TCAAGAATATGAGGCGGCTG-3' and reverse primer (1356-1375 in genome) 5'-TGGACGGGCTTGAT-GATTGG-3' for CVS11-N (207 bp amplicon), and forward primer 5'-TGACAGGATGCAGAAGGAGA-3' and reverse primer 5'-GCTGGAAGGTGGACAGTGAG-3' for β-actin (86 bp amplicon). The PCR was carried out on an Mx 3000P System. The relative expression values of N gene were normalized to the expression value of the β-actin gene.

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