Selected article for: "cdna fragment and single pcr"
Author: Chen, Wenbing; Li, Shousong; Shao, Biying; Zheng, Teng; Jiang, Shuxun; Huang, Xiaorong; Cai, Kaizhen; Zhang, Zhideng
Title: Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
  • Document date: 2016_11_28
    • ID: r2upa4o0_8
    Snippet: Using three primer pairs listed in Table 1 , the combination of the three target cDNA fragments with the size of 121, 182 and 302 bp was amplified by triplex PCR in simulated positive samples (Lane 15 in Figure 1 ). No cDNA fragment band was found in the relevant negative control with ddH 2 O (Lane 14 in Figure 1 ). Figure 1 showed that the PCR results were consistent in single PCR, duplex PCR, and triplex PCR. It indicated that the multiplex P.....
    Document: Using three primer pairs listed in Table 1 , the combination of the three target cDNA fragments with the size of 121, 182 and 302 bp was amplified by triplex PCR in simulated positive samples (Lane 15 in Figure 1 ). No cDNA fragment band was found in the relevant negative control with ddH 2 O (Lane 14 in Figure 1 ). Figure 1 showed that the PCR results were consistent in single PCR, duplex PCR, and triplex PCR. It indicated that the multiplex PCR technique could be applied to detect the SARS-CoV.

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