Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer Document date: 2020_1_1
ID: tymoeyoo_78
Snippet: The vectors pABG and pABL (both constructed in our laboratory) were used to express cetuximab and its mutants. The recombinant light and heavy chain vectors were cotransfected into FreeStyle HEK293T cells (Invitrogen) for instantaneous expression of the variant mAbs. Antibodies secreted in the supernatant were purified using a HiTrap Protein A FF 1-ml column (GE Healthcare)......
Document: The vectors pABG and pABL (both constructed in our laboratory) were used to express cetuximab and its mutants. The recombinant light and heavy chain vectors were cotransfected into FreeStyle HEK293T cells (Invitrogen) for instantaneous expression of the variant mAbs. Antibodies secreted in the supernatant were purified using a HiTrap Protein A FF 1-ml column (GE Healthcare).
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