Author: Han, Baek-Sang; Jang, Ho-Young; Racine, Trina; Qiu, Xiangguo; Sin, Jeong-Im
Title: Purification and characterization of monoclonal IgG antibodies recognizing Ebola virus glycoprotein Document date: 2018_7_31
ID: v5oh0haa_21
Snippet: HEK293-GP or HEK293 cells (5×10 5 ) were reacted at 4°C for 30 minutes with 2 μL of sera or 1 μg of the purified IgG antibodies in fluorescence-activated cell sorting (FACS) buffer (PBS+1% bovine serum albumin). This was followed by reaction at 4°C for 30 minutes with fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG (whole molecule) or anti-mouse IgG (Fc). In each step, the cells were washed twice with FACS buffer. The FITC-conjug.....
Document: HEK293-GP or HEK293 cells (5×10 5 ) were reacted at 4°C for 30 minutes with 2 μL of sera or 1 μg of the purified IgG antibodies in fluorescence-activated cell sorting (FACS) buffer (PBS+1% bovine serum albumin). This was followed by reaction at 4°C for 30 minutes with fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG (whole molecule) or anti-mouse IgG (Fc). In each step, the cells were washed twice with FACS buffer. The FITC-conjugated anti-mouse IgG (whole molecule) and anti-mouse IgG (Fc) were purchased from Sigma-Aldrich and Abcam (Cambridge, UK), respectively. Finally, the cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences, San Diego, CA, USA).
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