Author: SUZUKI, Kazuya; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens Document date: 2016_10_30
ID: tg4qupbm_8
Snippet: Because most retroviruses do not produce obvious CPE and persistently infect cultivated cells in vitro, virus neutralization tests are not easy for their serological tests, and ELISA and AGID are used for the antibody survey. Because lentiviruses are not oncogenic and do not malignantly transform cells as oncoviruses do, an established cell line is required for persistent production of a lentivirus. Because ZZ-R cells were shown to continuously r.....
Document: Because most retroviruses do not produce obvious CPE and persistently infect cultivated cells in vitro, virus neutralization tests are not easy for their serological tests, and ELISA and AGID are used for the antibody survey. Because lentiviruses are not oncogenic and do not malignantly transform cells as oncoviruses do, an established cell line is required for persistent production of a lentivirus. Because ZZ-R cells were shown to continuously release MVV antigens, this cell line may be used to produce large amounts of a viral antigen for serological tests.
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