Author: FAZ, Mirna; MARTÍNEZ, José Simón; QUIJANO-HERNÁNDEZ, Israel; FAJARDO, Raúl
Title: Reliability of clinical diagnosis and laboratory testing techniques currently used for identification of canine parvovirus enteritis in clinical settings Document date: 2016_11_6
ID: z3in6bi2_7
Snippet: The reaction mix contained 1 µl of GoTaq® Flexi DNA Polymerase 5 U/µl (Promega), 5 µl of GoTaq® Flexi buffer 5X Green, 3 µl of MgCl 2 25 mM, 4 µl dNTP's 200 µM, 2 µl of primers, 10 µl of DNA with a final concentration of 100 ng and 23 µl of nuclease free water. The reaction was carried out under the following amplification conditions: 1 cycle at 94°C for 5 min, followed by 35 cycles at 94°C for 30 sec, 50°C for 45 sec, 72°C for 1 m.....
Document: The reaction mix contained 1 µl of GoTaq® Flexi DNA Polymerase 5 U/µl (Promega), 5 µl of GoTaq® Flexi buffer 5X Green, 3 µl of MgCl 2 25 mM, 4 µl dNTP's 200 µM, 2 µl of primers, 10 µl of DNA with a final concentration of 100 ng and 23 µl of nuclease free water. The reaction was carried out under the following amplification conditions: 1 cycle at 94°C for 5 min, followed by 35 cycles at 94°C for 30 sec, 50°C for 45 sec, 72°C for 1 min and 1 cycle at 72°C for 5 min. Afterwards, 1 µl of the product of this reaction was used as a DNA template for the nesting procedure, and primers and amplification conditions were the same for 275 bp fragment.
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