Selected article for: "forward primer and primer forward primer"
Author: Lee, Jee Young; Jeon, Yongduk; Ahn, Mi Young; Ann, Hea Won; Jung, In Young; Jung, Wooyong; Kim, Moo Hyun; Ahn, Jin Young; Song, Je Eun; Kim, Yong Chan; Oh, Dong Hyun; Kim, Eun Jin; Jeong, Su Jin; Ku, Nam Su; Kim, Hyunsoo; Lee, Kyungwon; Kim, June Myung; Choi, Jun Yong
Title: An Imported Case of Brucella melitensis Infection in South Korea
  • Document date: 2017_11_14
    • ID: sc411pol_12
    Snippet: Analysis using 16s rRNA sequencing was used to confirm the presence of Brucella spp.. We used a forward primer of 5'-TTG-GAGAGTTTGATCCTGGCTC-3' and a reverse primer of 5'-GGCGTGGACTTCCAGGGTATCT-3' . Sequencing was commissioned by Macrogen. Blast and EzTaxon were used to analyze the sequence. According to Blast analysis, a 99.6% equivalence (720 of 723 bp) was found with several subspecies of Brucella (B. abortus, Brucella suis quencing the omp31 .....
    Document: Analysis using 16s rRNA sequencing was used to confirm the presence of Brucella spp.. We used a forward primer of 5'-TTG-GAGAGTTTGATCCTGGCTC-3' and a reverse primer of 5'-GGCGTGGACTTCCAGGGTATCT-3' . Sequencing was commissioned by Macrogen. Blast and EzTaxon were used to analyze the sequence. According to Blast analysis, a 99.6% equivalence (720 of 723 bp) was found with several subspecies of Brucella (B. abortus, Brucella suis quencing the omp31 gene. By PCR assay targeting the BCSP31 gene, Brucella spp. was confirmed. B. melitensis was confirmed by using PCR assay targeting the IS711 locus (Fig. 3 ).

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