Author: Nicola Clementi; Elena Criscuolo; Roberta Antonia Diotti; Roberto Ferrarese; Matteo Castelli; Roberto Burioni; Massimo Clementi; Nicasio Mancini
Title: Combined prophylactic and therapeutic use maximizes hydroxychloroquine anti-SARS-CoV-2 effects in vitro Document date: 2020_3_31
ID: fhy2z49t_8
Snippet: Viral RNA Mini Kit following manufacturers' instructions. Reverse transcription and subsequent amplification were performed using random hexamer primers. The amplicons were sequenced on Illumina MiSeq NGS platform (Illumina, San Diego, CA, USA). Amplicon purification and quantification were performed by Agencourt AMPure XP (Beckman Coulter, Villepinte, France) and Qubit dsDNA Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) author/funder. Al.....
Document: Viral RNA Mini Kit following manufacturers' instructions. Reverse transcription and subsequent amplification were performed using random hexamer primers. The amplicons were sequenced on Illumina MiSeq NGS platform (Illumina, San Diego, CA, USA). Amplicon purification and quantification were performed by Agencourt AMPure XP (Beckman Coulter, Villepinte, France) and Qubit dsDNA Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) author/funder. All rights reserved. No reuse allowed without permission.
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