Author: Goktas, Safak; Sirin, Mumtaz Cem
Title: Prevalence and Seasonal Distribution of Respiratory Viruses During the 2014 - 2015 Season in Istanbul Document date: 2016_8_22
ID: uhjftet5_7
Snippet: A total of 845 nasopharyngeal swab specimens were obtained from patients with a clinical pre-diagnosis of ARTI between September 1, 2014, and August 31, 2015. The specimens were transferred from healthcare institutions in Istanbul to Gelisim Medical laboratories, and analyzed directly or stored at -80°C until tested. The samples were analyzed for respiratory viruses and bacteria by using the RespiFinder Smart 22 assay (PathoFinder BV, the Nether.....
Document: A total of 845 nasopharyngeal swab specimens were obtained from patients with a clinical pre-diagnosis of ARTI between September 1, 2014, and August 31, 2015. The specimens were transferred from healthcare institutions in Istanbul to Gelisim Medical laboratories, and analyzed directly or stored at -80°C until tested. The samples were analyzed for respiratory viruses and bacteria by using the RespiFinder Smart 22 assay (PathoFinder BV, the Netherlands). Each sample was simultaneously tested for the following 22 pathogens: IFV-A, IFV-A (H1N1)pdm09, IFV-B, PIV-1, PIV-2, PIV-3, PIV-4, RSV-A, RSV-B, HMPV, rhinovirus/enterovirus (RV/EV), HBoV, AdV, HCoV NL63, HCoV HKU1, HCoV 229E, HCoV OC43, Bordetella pertussis (BP), Chlamydophila pneumoniae (CP), Legionella pneumophila (LP), and Mycoplasma pneumoniae (MP). First, viral and bacterial nucleic acids (DNA or RNA) were extracted according to the kit protocol. Next, amplification, detection, and data analysis were performed with the Rotor-Gene 6000 realtime PCR system (Qiagen, Germany) according to the manufacturer's instructions (11, 12) .
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