Title: Research Communications of the 24th ECVIM-CA Congress Document date: 2015_1_10
ID: r59usk02_298
Snippet: With owner consent, treatment-na€ ıve tumour sections were collected from a dog with disseminated HS that was euthanased. Tumour tissue was assessed with immunohistochemistry (IHC) using antibodies against canine CD18, CD3, and PAX-5 to support the diagnosis of histiocytic sarcoma. Primary cell cultures (HSCs), established from the tumour were cultured and maintained in modified Eagle's medium with 10% fetal bovine serum, L-glutamine, penicill.....
Document: With owner consent, treatment-na€ ıve tumour sections were collected from a dog with disseminated HS that was euthanased. Tumour tissue was assessed with immunohistochemistry (IHC) using antibodies against canine CD18, CD3, and PAX-5 to support the diagnosis of histiocytic sarcoma. Primary cell cultures (HSCs), established from the tumour were cultured and maintained in modified Eagle's medium with 10% fetal bovine serum, L-glutamine, penicillin and streptomycin, in standard conditions. HSCs were characterised by alpha naphthyl acetate esterase (ANAE) and lysozyme staining while PCR was used to detect cell markers CD1a, CD11c, MHC II, CD204, CCR2, E-cadherin, and CD4. Cell surface markers were compared to an established canine HS cell line (DH82). Phagocytic activity of HSC cells was assessed using cellular uptake of carboxylated fluorescent beads and documented using flow cytometry and fluorescent microscopy.
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