Author: Gunn, Michael D.; Kyuwa, Shigeru; Tam, Carmen; Kakiuchi, Terutaka; Matsuzawa, Akio; Williams, Lewis T.; Nakano, Hideki
Title: Mice Lacking Expression of Secondary Lymphoid Organ Chemokine Have Defects in Lymphocyte Homing and Dendritic Cell Localization Document date: 1999_2_1
ID: sz28ar3t_9
Snippet: Immunohistochemistry. Tissue samples of spleen and LNs were frozen in OCT, and 10-m cryostat sections were prepared. Sections were fixed in acetone for 10 min, air dried, blocked with PBS/5% normal goat serum for 30 min, and incubated with the indicated primary antibodies for 1 h. Slides were then washed in PBS, incubated with either SA-AP and anti-rat HRP or anti-rat AP and SA-HRP for 30 min, and developed with 3,3'-diaminobenzidine (DAB) follow.....
Document: Immunohistochemistry. Tissue samples of spleen and LNs were frozen in OCT, and 10-m cryostat sections were prepared. Sections were fixed in acetone for 10 min, air dried, blocked with PBS/5% normal goat serum for 30 min, and incubated with the indicated primary antibodies for 1 h. Slides were then washed in PBS, incubated with either SA-AP and anti-rat HRP or anti-rat AP and SA-HRP for 30 min, and developed with 3,3'-diaminobenzidine (DAB) followed by Vector Red (Vector Labs) according to the manufacturer's instructions.
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