Selected article for: "amplification curve and efficiency amplification"
Author: Yu Jin Jung; Gun-Soo Park; Jun Hye Moon; Keunbon Ku; Seung-Hwa Beak; Seil Kim; Edmond Changkyun Park; Daeui Park; Jong-Hwan Lee; Cheol Woo Byeon; Joong Jin Lee; Jin-Soo Maeng; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Min Jun Lee; Hong Gi Kim
Title: Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
  • Document date: 2020_2_27
    • ID: 2hyiped2_15
    Snippet: The Ct value was not produced from negative control, indicating the reaction was done aseptically. The standard curve from E gene primer-probe set also showed the reaction was done accordingly. The R 2 value of the standard curve was 0.999 and the calculated amplification efficiency was 101.6%. These indicated that the qRT-PCR reaction was done in optimal condition. The viral concentration of supernatant and cell lysate was determined by E gene-b.....
    Document: The Ct value was not produced from negative control, indicating the reaction was done aseptically. The standard curve from E gene primer-probe set also showed the reaction was done accordingly. The R 2 value of the standard curve was 0.999 and the calculated amplification efficiency was 101.6%. These indicated that the qRT-PCR reaction was done in optimal condition. The viral concentration of supernatant and cell lysate was determined by E gene-based assay ( Table 2) .

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