Selected article for: "comparative analysis and qrt PCR SARS comparative analysis"
Author: Yu Jin Jung; Gun-Soo Park; Jun Hye Moon; Keunbon Ku; Seung-Hwa Beak; Seil Kim; Edmond Changkyun Park; Daeui Park; Jong-Hwan Lee; Cheol Woo Byeon; Joong Jin Lee; Jin-Soo Maeng; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Min Jun Lee; Hong Gi Kim
Title: Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
  • Document date: 2020_2_27
    • ID: 2hyiped2_20
    Snippet: Nevertheless, there have been no comparative results of the current qRT-PCR analysis for the molecular diagnosis of SARS-CoV-2. In the present study, the first comparative analysis of various primer-probe sets targeting RdRp/Orf1 and N region of SARS-CoV-2 was performed by qRT-PCR for the laboratory confirmation. In the case of targeting RdRp/Orf1 region, ORF1ab (China) set might be the most sensitive than other sets. 2019-nCoV_N2, N3 (USA), and .....
    Document: Nevertheless, there have been no comparative results of the current qRT-PCR analysis for the molecular diagnosis of SARS-CoV-2. In the present study, the first comparative analysis of various primer-probe sets targeting RdRp/Orf1 and N region of SARS-CoV-2 was performed by qRT-PCR for the laboratory confirmation. In the case of targeting RdRp/Orf1 region, ORF1ab (China) set might be the most sensitive than other sets. 2019-nCoV_N2, N3 (USA), and NIID_2019-nCOV_N (Japan) sets may be recommended for the sensitive qRT-PCR assay of N region. Therefore, the appropriate combination from ORF1ab (China), 2019-nCoV_N2, N3 (USA), and NIID_2019-nCOV_N (Japan) sets should be selected for the sensitive and reliable laboratory confirmation of SARS-CoV-2.

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