Title: Research Communications of the 24th ECVIM-CA Congress Document date: 2015_1_10
ID: r59usk02_552
Snippet: A validated method was produced, with a lower limit of detection of 0.009 mg/mL and an lower limit of quantification of 0.017 mg/mL. The upper limit of quantification was 2.16 mg/mL. The standard curve had excellent linearity (R 2 = 0.993). Maximum inaccuracy was less than 11.5% of the true value, except at LLOQ, where it was within 24.2%. Average within day variability was less than 13.1% at all levels, while between day variability was less tha.....
Document: A validated method was produced, with a lower limit of detection of 0.009 mg/mL and an lower limit of quantification of 0.017 mg/mL. The upper limit of quantification was 2.16 mg/mL. The standard curve had excellent linearity (R 2 = 0.993). Maximum inaccuracy was less than 11.5% of the true value, except at LLOQ, where it was within 24.2%. Average within day variability was less than 13.1% at all levels, while between day variability was less than 5.6%, except at LLOQ, where it was less than 15.4%. Agreement between the results obtained by measurement with HPCE and MS was good (bias 1.3%, lower and upper limits of agreement of -15.4 and 18.0%, respectively). Method specificity was confirmed by the absence of matrix effect in six serum specimen obtained from clinical dogs. Clinical samples were analysed and GFR reported with a 3 day turnaround time.
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